故事讲述被江湖各派推举為“武林之尊”的修德武馆,被胸怀野心的朱天翔(罗烈 饰)踢馆捣乱,幸得少主人徐近武(张翼 饰)打退。天翔得知韩氏三姐妹若兰(沉依 饰)、如玫(林嘉 饰)、晓莲(潘迎紫 饰)家藏威力惊人的玄铁神剑,若能夺得,武馆必败,遂以花言巧语骗得神剑……The plot follows the titular sisters, Ruo Lien, Ruo Lan and Ruo Mei whose clan protects the famous mighty steel sword, a powerful weapon much sought after by their rivals, in particular the nasty Chu Tien Hsiang (played by the legendary actor Lo Lieh, known at the time for being the studio's first international superstar, appearing in the likes of "King Boxer" and "Lady Hermit") who aims to use it to become number one in the martial arts world. Infiltrating the trio with his cunning schemes, he seizes the blade and sets about eliminating its rightful owners as the girls band together to bring him to justice.